Anti-N WASP (phospho Y256) antibody (ab23395)
Overview
- Product nameAnti-N WASP (phospho Y256) antibodySee all N WASP primary antibodies ...
- DescriptionRabbit polyclonal to N WASP (phospho Y256)
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Phospho N WASP (Tyr 256) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around tyrosine 256 of human N WASP. The human WASP sequence has a similar peptide sequence surrounding tyrosine 291.
- Positive controlPervanadate treated A431, HeLa, Jurkat and endothelial cells for SDS PAGE immunoblots.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide.
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was cross absorbed to phosphotyrosine then affinity purified using phospho N WASP (Tyr 256) peptide.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab23395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa). The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine. |
| ELISA | ELISA: 1/20000. |
Target
- FunctionRegulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
- Sequence similaritiesContains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 2 WH2 domains. - Cellular localizationCytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.
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Database links
- Entrez Gene: 8976 Human
- Entrez Gene: 8976 Human
- Omim: 605056 Human
- SwissProt: O00401 Human
- SwissProt: O00401 Human
- Unigene: 143728 Human
- Unigene: 83916 Human
Target information above from: UniProt accession
O00401
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- DKFZp779G0847 antibodyMGC48327 antibodyN-WASP antibody
- Neural Wiskott Aldrich syndrome protein antibodyNeural Wiskott-Aldrich syndrome protein antibodyNWASP antibodyWASL antibodyWASL_HUMAN antibodyWiskott Aldrich syndrome gene like antibodyWiskott Aldrich syndrome gene like protein antibodyWiskott Aldrich syndrome like antibodyWiskottAldrich syndrome like antibody
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Anti-N WASP (phospho Y256) antibody images
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Western blot - N WASP (phospho Y256) antibody (ab23395)All lanes : Anti-N WASP (phospho Y256) antibody (ab23395) at 1/10000 dilution
Lane 1 : Control A431 cells 20ug/lane
Lane 2 : Pervanadate-treated A431 cells 20 ug/lane
Predicted band size : 55 kDa
References for Anti-N WASP (phospho Y256) antibody (ab23395)
This product has been referenced in:
- Rajput C et al. Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions. J Biol Chem 288:4241-50 (2013). WB ; Human . Read more (PubMed: 23212915) »
- Chacón MR et al. Focal adhesion kinase regulates actin nucleation and neuronal filopodia formation during axonal growth. Development 139:3200-10 (2012). WB . Read more (PubMed: 22872087) »
