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Anti-N acetylglucosamine kinase antibody (ab37579) at 1/2000 dilution + E coli-derived fusion protein at 2.5 µg
Secondary
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size : 37 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Predicted and actual molecular weights differ due to the use of a fusion protein.
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