The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/200.
1/50 - 1/200.
1/500 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for N myc interactor (green) using ab224675 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
has not yet been referenced specifically in any publications.
Publishing research using ab224675? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Get resources and offers direct to your inboxSign up