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Western blot - N myc interactor antibody (ab28743)
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Product Name
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N myc interactor antibody
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See all N myc interactor antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to N myc interactor
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Immunogen
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Synthetic peptide derived from residues 66 - 115 of human N myc interactor.
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Reacts with
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Hu
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Specificity
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ab28743 recognises NMYC interactor (NMI).
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
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Recommended dilutions
ELISA: 1/62500.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this N myc interactor antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for N myc interactor antibody images, references, products related to ab28743 and other tools.
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N myc interactor antibody images:
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 Western blot - N myc interactor antibody (ab28743)
N myc interactor antibody (ab28743) at 1.25 µg/ml + Fetal liver lysate
Secondary
HRP conjugated anti-Rabbit IgG
Predicted band size : 35 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%.
Suggested dilution for HRP-conjugated secondary antibody: 1/50000 - 100000
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Search PubMed (MEDLINE) for references to N myc interactor
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N myc interactor antibody - more information
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Customer reviews (feedback) regarding N myc interactor antibody |
Customer FAQs regarding N myc interactor antibody |
Protocols for N myc interactor antibody |
Price and availability of products related to N myc interactor antibody |
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N myc interactor antibody - related products:
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N myc interactor antibody - other tools:
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Contact Abcam with a Technical Enquiry about N myc interactor antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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