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Anti-N myc interactor antibody (ab28743)

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Overview

Product name

Anti-N myc interactor antibody
See all N myc interactor products (7) ...

Description

Rabbit polyclonal to N myc interactor

Specificity

ab28743 recognises NMYC interactor (NMI).

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Cow

Immunogen

Synthetic peptide derived from residues 66 - 115 of human N myc interactor.

Read Abcam's proprietary immunogen policy

.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

  • Western blot - N myc interactor antibody (ab28743)Western blot - N myc interactor antibody (ab28743) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).

  • ELISA

     ELISA: 1/62500

    ELISA: 1/62500

Target

Relevance

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.

Cellular localization

Cytoplasmic

Alternative names

  • N myc and STAT interactor antibody
  • N myc and STAT interactor antibody
  • N myc interactor antibody
  • NMI antibody
  • NMYC and STAT interactor antibody
  • NMYC interactor antibody
see all

Database links

Anti-N myc interactor antibody images:

  Western blot - N myc interactor antibody (ab28743)

Western blot - N myc interactor antibody (ab28743)

Anti-N myc interactor antibody (ab28743) at 1.25 µg/ml + Fetal liver lysate

Secondary
HRP conjugated anti-Rabbit IgG

Predicted band size : 35 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)


Gel concentration: 12%. Suggested dilution for HRP-conjugated secondary antibody: 1/50000 - 100000

References for Anti-N myc interactor antibody (ab28743)

ab28743 has not yet been referenced specifically in any publications.

Publishing research using ab28743? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"