|
|
|
|
Western blot - N myc interactor antibody (ab70210)
(enlarge)
|
|
|
|
|
Product Name
|
N myc interactor antibody
|
| |
See all N myc interactor antibodies (6)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to N myc interactor
|
|
Immunogen
|
Synthetic peptide corresponding to a region between C terminal residues 250 and 307 of human N myc interactor. (NP_004679.1)
|
|
Reacts with
|
Hu
|
|
Does not react with
(species key)
|
Ms
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: 1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Whole cell lysate from HeLa cells.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
|
|
Relevance
|
NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, TBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this N myc interactor antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for N myc interactor antibody images, references, products related to ab70210 and other tools.
|
| |
N myc interactor antibody images:
|
 Western blot - N myc interactor antibody (ab70210)
All lanes : N myc interactor antibody (ab70210) at 0.04 µg/ml
Lane 1 : Whole
cell lysate from HeLa cells at 5 µg
Lane 2 : Whole
cell lysate from HeLa cells at 15 µg
Lane 3 : Whole
cell lysate from HeLa cells at 50
Lane 4 : Whole
cell lysate from 293T cells at 50
Lane 5 : Whole
cell lysate from NIH3T3 cells at 50
Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
|
| |
| |
|
Search PubMed (MEDLINE) for references to N myc interactor
|
| |
N myc interactor antibody - more information
|
Customer reviews (feedback) regarding N myc interactor antibody |
Customer FAQs regarding N myc interactor antibody |
Protocols for N myc interactor antibody |
Price and availability of products related to N myc interactor antibody |
| |
N myc interactor antibody - related products:
|
|
|
| |
N myc interactor antibody - other tools:
|
Contact Abcam with a Technical Enquiry about N myc interactor antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
