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Anti-N myc interactor antibody (ab70210)

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Overview

Product name

Anti-N myc interactor antibody
See all N myc interactor products (7) ...

Description

Rabbit polyclonal to N myc interactor

Specificity

We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide corresponding to a region between C terminal residues 250 and 307 of human N myc interactor. (NP_004679.1)

Positive control

Whole cell lysate from HeLa cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

  • Western blot - N myc interactor antibody (ab70210)Western blot - N myc interactor antibody (ab70210) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab70210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/2000 - 1/10000.Det...Read more →

    WB: 1/2000 - 1/10000.Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).

Application notes

Is unsuitable for or IP.

Target

Relevance

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.

Cellular localization

Cytoplasmic

Alternative names

  • N myc and STAT interactor antibody
  • N myc interactor antibody
  • NMI antibody
  • NMYC and STAT interactor antibody
  • NMYC interactor antibody
see all

Database links

Anti-N myc interactor antibody images:

  Western blot - N myc interactor antibody (ab70210)

Western blot - N myc interactor antibody (ab70210)

All lanes : Anti-N myc interactor antibody (ab70210) at 0.04 µg/ml

Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50
Lane 4 : Whole cell lysate from 293T cells at 50
Lane 5 : Whole cell lysate from NIH3T3 cells at 50


Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)


Exposure time : 30 seconds

References for Anti-N myc interactor antibody (ab70210)

ab70210 has not yet been referenced specifically in any publications.

Publishing research using ab70210? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"