Recombinant
RabMAb

Anti-N2,N2-dimethylguanosine antibody [EPR-19838-40] (ab211488)

Overview

  • Product name
    Anti-N2,N2-dimethylguanosine antibody [EPR-19838-40]
  • Description
    Rabbit monoclonal [EPR-19838-40] to N2,N2-dimethylguanosine
  • Tested applications
    Suitable for: IP, ELISAmore details
  • Immunogen

    Chemical/ Small Molecule within N2,N2-dimethylguanosine. The exact sequence is proprietary.

  • Positive control
    • IP: Biotin-mN-mN-mN-mN-mN-(m2,2G)*-mN-mN-mN-mN-mN, * - phosphorothioate protection.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR-19838-40
  • Isotype
    IgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab211488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

0.2 µg coated onto 50 µL Dynabeads® sheep-anti-rabbit IgG.

ELISA Use a concentration of 0.005 - 4 µg/ml.

Target

  • Alternative names
    • Di-m2G antibody
    • m2,2G antibody
    • m22G antibody
    • N2,N2 dimethylguanosine antibody

Images

  • The IP was performed in a U-bottom non-adsorbing propylene 96-well plate. 0.2 µg of ab211488 was conjugated to 50 µL Dynabeads® Sheep anti-Rabbit IgG for 1 hour at RT. 5 µM unmodified/modified oligonucleotides were added to the samples containing antibody/beads complexes and incubated with agitation for 1 hour at RT. After washes, Peroxidase-conjugated Streptavidin was added at 1/1000 dilution and the mix was incubated with agitation for 1 hour at RT. After adding ECL substrate the obtained results were read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ. 

    Lane 1: Buffer only.

    Lane 2: 5 µM Modified oligonucleotide Biotin-mN-mN-mN-mN-mN-(m2,2G)*-mN-mN-mN-mN-mN.

    Lane 3: 5 µM Unmodified oligonucleotide Biotin-mN-mN-mN-mN-mN-(G)*-mN-mN-mN-mN-mN.

    N - equimolar mixture of (A/U/G/C); m - 2’O methyl protection; * - phosphorothioate protection

     

    Exposure time: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • BSA-conjugated m2,2G-modified and unmodified nucleotides were coated onto wells of the plate. ELISA was performed on 1.0 µg/ml of antigen using ab211488 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

References

ab211488 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211488.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up