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Chemical/ Small Molecule within N2,N2-dimethylguanosine. The exact sequence is proprietary.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab211488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
0.2 µg coated onto 50 µL Dynabeads® sheep-anti-rabbit IgG.
|ELISA||Use a concentration of 0.005 - 4 µg/ml.|
The IP was performed in a U-bottom non-adsorbing propylene 96-well plate. 0.2 µg of ab211488 was conjugated to 50 µL Dynabeads® Sheep anti-Rabbit IgG for 1 hour at RT. 5 µM unmodified/modified oligonucleotides were added to the samples containing antibody/beads complexes and incubated with agitation for 1 hour at RT. After washes, Peroxidase-conjugated Streptavidin was added at 1/1000 dilution and the mix was incubated with agitation for 1 hour at RT. After adding ECL substrate the obtained results were read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.
Lane 1: Buffer only.
Lane 2: 5 µM Modified oligonucleotide Biotin-mN-mN-mN-mN-mN-(m2,2G)*-mN-mN-mN-mN-mN.
Lane 3: 5 µM Unmodified oligonucleotide Biotin-mN-mN-mN-mN-mN-(G)*-mN-mN-mN-mN-mN.
N - equimolar mixture of (A/U/G/C); m - 2’O methyl protection; * - phosphorothioate protection
Exposure time: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
BSA-conjugated m2,2G-modified and unmodified nucleotides were coated onto wells of the plate. ELISA was performed on 1.0 µg/ml of antigen using ab211488 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.
ab211488 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"