Anti-N6-methyladenosine antibody (ab151230)

Overview

  • Product nameAnti-N6-methyladenosine antibody
  • Description
    Rabbit polyclonal to N6-methyladenosine
  • Tested applicationsPepArr more details
  • Species reactivity
    Reacts with: A wide range of mammals
  • Immunogen

    Chemical/ Small Molecule corresponding to N6-methyladenosine (methyl ). KLH-conjugated N6-methyladenosine.

  • Positive control
    • ab151230 was tested in protein array against RNA oligomers; N6-methyladenosine and unmodified adenosine. 2Ome-RNA oligomers carry a methyl group at the 2’-OH residue of the ribose molecule, making them resistant to most ribonucleases.
  • General notes

    N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.

    The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.4
    Constituent: PBS

    PBS prepared using DEPC-treated water.
    The use of RNase is recommended
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab151230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
PepArr Use a concentration of 2 µg/ml.

Anti-N6-methyladenosine antibody images

  • All batches of ab151230 are tested in Protein Array against N6-methyladenosine and unmodified adenosine. Six dilutions of each oligomer are printed on to the Protein Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.

    2Ome-m6A - N6-methyladenosine

    2Ome-m6A_unmod - unmodified adenosine

References for Anti-N6-methyladenosine antibody (ab151230)

ab151230 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"