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Anti-NAALADL2 antibody (ab76801) at 1 µg/ml + immunogen (GST-tagged, 100 residues recombinant fragment from Human NAALADL2) at 0.1 µg
Secondary
Goat anti-mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 89 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
MW of GST tag alone: 26 kDa
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