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Anti-NAALADL2 antibody (ab76801)

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Overview

Product name

Anti-NAALADL2 antibody

Description

Mouse monoclonal to NAALADL2

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Recombinant Fragment

Predicted to work with

Horse, Dog, Human, Chimpanzee

Immunogen

GST-tagged recombinant fragment: DPKERAPIRI RMLNDILQDM EKSFLVKQAP PGFYRNILYH LDEKTSRFSI LIEAWEHCKP LASNETLQEA LSEVLNSINS AQVYFKAGLD VFKSVLDGKN, corresponding to amino acids 696-795 of Human NAALADL2 (NP_996898)

DPKERAPIRI RMLNDILQDM EKSFLVKQAP PGFYRNILYH LDEKTSRFSI LIEAWEHCKP LASNETLQEA LSEVLNSINS AQVYFKAGLD VFKSVLDGKN

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1X PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Isotype

IgG2a

Light chain type

kappa

Research areas

Cell Biology >> Other Antibodies >> Other Antibodies

  • Western blot - NAALADL2 antibody (ab76801)Western blot - NAALADL2 antibody (ab76801) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight of endogenous protein: 89 kDa.
Note: this antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

NAALADL2 belongs to the peptidase M28 family, M28B subfamily. However, it may be catalytically inactive due to the lack of the conserved zinc-binding and active sites. It is expressed at higher level in kidney and placenta. In embryo, it is mainly confined to duodenal and stomach endoderm, mesonephros, metanephros and pancreas. There are two named isoforms. There are two named isoforms.

Cellular localization

Membrane; Single-pass type II membrane protein.

Alternative names

  • Inactive N acetylated alpha linked acidic dipeptidase like protein 2 antibody
  • N acetylated alpha linked acidic dipeptidase like 2 antibody
  • NAALADase L2 antibody
  • NAALADL 2 antibody

Database links

Anti-NAALADL2 antibody images:

  Western blot - NAALADL2 antibody (ab76801)

Western blot - NAALADL2 antibody (ab76801)

Anti-NAALADL2 antibody (ab76801) at 1 µg/ml + immunogen (GST-tagged, 100 residues recombinant fragment from Human NAALADL2) at 0.1 µg

Secondary
Goat anti-mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

Predicted band size : 89 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)


MW of GST tag alone: 26 kDa

References for Anti-NAALADL2 antibody (ab76801)

ab76801 has not yet been referenced specifically in any publications.

Publishing research using ab76801? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"