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Western blot - NAB1 antibody (ab50381)
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Immunohistochemistry (Paraffin-embedded sections) - NAB1 antibody (ab50381)
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Product Name
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NAB1 antibody
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See all NAB1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NAB1
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Immunogen
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Synthetic peptide corresponding to a region of Human NAB1
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Titre using peptide based assay 1/62500.
IHC-P: Use at a concentration of 16 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human intestine, transfected 293T cell lysate
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Cellular localization
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Nuclear
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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NAB1 belongs to the NAB family and acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. It has two named isomers. Homomultimers may associate with EGR1 bound to DNA.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NAB1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NAB1 antibody images, references, products related to ab50381 and other tools.
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NAB1 antibody images:
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 Western blot - NAB1 antibody (ab50381)
NAB1 antibody (ab50381) at 1 µg/ml + Transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 54 kDa
Observed band size : 54 kDa
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
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Immunohistochemistry (Paraffin-embedded sections) - NAB1 antibody (ab50381)
Ab50381 at 16µg/ml staining epithelial cells of human intestinal villus; paraffin embedded; magnification x400
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Search PubMed (MEDLINE) for references to NAB1
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NAB1 antibody - more information
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Customer reviews (feedback) regarding NAB1 antibody |
Customer FAQs regarding NAB1 antibody |
Protocols for NAB1 antibody |
Price and availability of products related to NAB1 antibody |
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NAB1 antibody - related products:
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NAB1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NAB1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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