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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> ER Translocation
Overview
Product name
Anti-NACA2 antibody
Description
Mouse polyclonal to NACA2
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length human NACA2 (NP_954984.1).
Positive control
NACA2 transfected 293T lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Immunology >> Immune System Diseases >> Autoimmune
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> ER Translocation
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Western blot - NACA2 antibody (ab69439)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa).
Target
Relevance
NACA2, Nascent polypeptide associated complex subunit alpha 2, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER) by binding to nascent polypeptide chains as they emerge from the ribosome and blocking their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. NACA2 also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites).
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- MGC71999 antibody
- Alpha NAC like antibody
- ANAC antibody
see all
Database links
- Entrez Gene: 342538 Human
- GenBank: NM_199290.3 Human
- Omim: 609274 Human
- SwissProt: Q9H009 Human
Anti-NACA2 antibody images:
Western blot - NACA2 antibody (ab69439)
All lanes : Anti-NACA2 antibody (ab69439) at 1/500 dilution
Lane 1 : NACA2 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 24 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
References for Anti-NACA2 antibody (ab69439)
ab69439 has not yet been referenced specifically in any publications.
Publishing research using ab69439? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"