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Western blot - NACA2 antibody (ab69439)
(enlarge)
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Product Name
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NACA2 antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to NACA2
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Immunogen
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Full length human NACA2 (NP_954984.1).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NACA2 transfected 293T lysate.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Immunology >> Immune System Diseases >> Autoimmune
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> ER Translocation
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Relevance
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NACA2, Nascent polypeptide associated complex subunit alpha 2, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER) by binding to nascent polypeptide chains as they emerge from the ribosome and blocking their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. NACA2 also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NACA2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NACA2 antibody images, references, products related to ab69439 and other tools.
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NACA2 antibody images:
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 Western blot - NACA2 antibody (ab69439)
All lanes : NACA2 antibody (ab69439) at 1/500 dilution
Lane 1 : NACA2 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 24 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to NACA2
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NACA2 antibody - more information
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Customer reviews (feedback) regarding NACA2 antibody |
Customer FAQs regarding NACA2 antibody |
Protocols for NACA2 antibody |
Price and availability of products related to NACA2 antibody |
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NACA2 antibody - related products:
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NACA2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NACA2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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