Recombinant Anti-NADSYN1 antibody [EPR10611] (ab171942)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10611] to NADSYN1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NADSYN1 antibody [EPR10611]
See all NADSYN1 primary antibodies -
Description
Rabbit monoclonal [EPR10611] to NADSYN1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa and 293T cell lysates; Human cervical carcinoma and testis tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10611 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 79 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 79 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
Target
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Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1. -
Sequence similarities
In the C-terminal section; belongs to the NAD synthetase family.
Contains 1 CN hydrolase domain. - Information by UniProt
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Database links
- Entrez Gene: 55191 Human
- Omim: 608285 Human
- SwissProt: Q6IA69 Human
- Unigene: 556986 Human
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Alternative names
- Glutamine dependent NAD(+) synthetase antibody
- Glutamine-dependent NAD(+) synthetase antibody
- NAD(+) synthase [glutamine hydrolyzing] antibody
see all
Images
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All lanes : Anti-NADSYN1 antibody [EPR10611] (ab171942) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 79 kDa -
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling NADSYN1 with ab171942 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NADSYN1 with ab171942 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling NADSYN1 with ab171942 at 1/50 dilution.
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Intracellular flow cytometric analysis ofpermeabilized HeLa cells labeling NADSYN1 with ab171942at 1/100 dilution (red)ocompared with a Rabbit IgG (negative) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab171942 has been referenced in 3 publications.
- Kudo K et al. Divergent metabolic responses dictate vulnerability to NAMPT inhibition in ovarian cancer. FEBS Lett 594:1379-1388 (2020). PubMed: 31950503
- Chowdhry S et al. NAD metabolic dependency in cancer is shaped by gene amplification and enhancer remodelling. Nature 569:570-575 (2019). PubMed: 31019297
- James EL et al. Replicatively senescent human fibroblasts reveal a distinct intracellular metabolic profile with alterations in NAD+ and nicotinamide metabolism. Sci Rep 6:38489 (2016). PubMed: 27924925