Anti-ND6 antibody (ab214224)
Key features and details
- Rabbit polyclonal to ND6
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ND6 antibody
See all ND6 primary antibodies -
Description
Rabbit polyclonal to ND6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human ND6 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P03923 -
Positive control
- Human breast cancer and rat brain tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
NADH Dehydrogenase subunit 6 (MTND6) is 1 of the 7 mitochondrial DNA (mtDNA) encoded subunits (MTND1, MTND2, MTND3, MTND4L, MTND4, MTND5, MTND6) included among the approximately 41 polypeptides of respiratory Complex I. Complex I accepts electrons from NADH, transfers them to ubiquinone (Coenzyme Q10), and uses the energy released to pump protons across the mitochondria inner membrane. MTND6 has been proposed to be a component of the iron-protein fragment. The predicted polypeptide has a molecular weight of 18.6 kD. Its apparent MW on SDS-polyacrylamide gels (PAGE) using Tris-glycine buffer is 16.7 kD, and the apparent MW on urea-phosphate gels is also close to the predicted molecular weight. -
Cellular localization
Mitochondrion membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 4541 Human
- Entrez Gene: 17722 Mouse
- Entrez Gene: 26203 Rat
- Omim: 516006 Human
- SwissProt: P03923 Human
- SwissProt: P03925 Mouse
- SwissProt: P03926 Rat
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Alternative names
- Mitochondrially encoded NADH dehydrogenase 6 antibody
- MT ND6 antibody
- mtND6 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling ND6 with ab214224 at 1/100 dilution, followed by secondary antibody detection and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling ND6 with ab214224 at 1/400 dilution, followed by secondary antibody detection and DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab214224 has not yet been referenced specifically in any publications.