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Products:Signal Transduction >> Metabolism >> Mitochondrial
Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab81212? Please let us know so that we can cite the reference in this datasheet
ab81212 has been referenced in 1 publications.
- Karamanlidis G et al. Defective DNA replication impairs mitochondrial biogenesis in human failing hearts. Circ Res 106:1541-8 (2010). PubMed: 20339121
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - NADH Dehydrogenase subunit 6 antibody (ab81212)
Anti-NADH Dehydrogenase subunit 6 antibody (ab81212) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 19 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)
IHC image of ab81212 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81212, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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