Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-NADH Dehydrogenase subunit 6 antibody
Description
Rabbit polyclonal to NADH Dehydrogenase subunit 6
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 110-159 (DGVVVVVNFN SVGSWMIYEG EGSGLIREDP IGAGALYDYG RWLVVVTGWT) of Human NADH Dehydrogenase subunit 6 (NP_536854).
DGVVVVVNFN SVGSWMIYEG EGSGLIREDP IGAGALYDYG RWLVVVTGWT
Positive control
Human fetal lung lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 19 kDa.
Target
Relevance
NADH Dehydrogenase subunit 6 (MTND6) is 1 of the 7 mitochondrial DNA (mtDNA) encoded subunits (MTND1, MTND2, MTND3, MTND4L, MTND4, MTND5, MTND6) included among the approximately 41 polypeptides of respiratory Complex I. Complex I accepts electrons from NADH, transfers them to ubiquinone (Coenzyme Q10), and uses the energy released to pump protons across the mitochondria inner membrane. MTND6 has been proposed to be a component of the iron-protein fragment. The predicted polypeptide has a molecular weight of 18.6 kD. Its apparent MW on SDS-polyacrylamide gels (PAGE) using Tris-glycine buffer is 16.7 kD, and the apparent MW on urea-phosphate gels is also close to the predicted molecular weight.
Cellular localization
Mitochondrion membrane; Multi-pass membrane protein.
Alternative names
- Mitochondrially encoded NADH dehydrogenase 6 antibody
- MT ND6 antibody
- mtND6 antibody
see all
Database links
- Entrez Gene: 4541 Human
- Omim: 516006 Human
- SwissProt: P03923 Human
Anti-NADH Dehydrogenase subunit 6 antibody images:
Western blot - NADH Dehydrogenase subunit 6 antibody (ab81212)
Anti-NADH Dehydrogenase subunit 6 antibody (ab81212) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 19 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)
IHC image of ab81212 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81212, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)
This product has been referenced in:
- Karamanlidis Get al. Defective DNA replication impairs mitochondrial biogenesis in human failing hearts. Circ Res 106:1541-8 (2010).Read more (PubMed: 20339121) »
See 1 publication for this product
Publishing research using ab81212? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"