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Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)

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Overview

Product name

Anti-NADH Dehydrogenase subunit 6 antibody

Description

Rabbit polyclonal to NADH Dehydrogenase subunit 6

Tested applications

IHC-P, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Chimpanzee

Immunogen

Synthetic peptide corresponding to a region within internal sequence amino acids 110-159 (DGVVVVVNFN SVGSWMIYEG EGSGLIREDP IGAGALYDYG RWLVVVTGWT) of Human NADH Dehydrogenase subunit 6 (NP_536854).

DGVVVVVNFN SVGSWMIYEG EGSGLIREDP IGAGALYDYG RWLVVVTGWT

Positive control

Human fetal lung lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - NADH Dehydrogenase subunit 6 antibody (ab81212)Western blot - NADH Dehydrogenase subunit 6 antibody (ab81212) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADH Dehydrogenase subunit 6 antibody (ab81212) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab81212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

NADH Dehydrogenase subunit 6 (MTND6) is 1 of the 7 mitochondrial DNA (mtDNA) encoded subunits (MTND1, MTND2, MTND3, MTND4L, MTND4, MTND5, MTND6) included among the approximately 41 polypeptides of respiratory Complex I. Complex I accepts electrons from NADH, transfers them to ubiquinone (Coenzyme Q10), and uses the energy released to pump protons across the mitochondria inner membrane. MTND6 has been proposed to be a component of the iron-protein fragment. The predicted polypeptide has a molecular weight of 18.6 kD. Its apparent MW on SDS-polyacrylamide gels (PAGE) using Tris-glycine buffer is 16.7 kD, and the apparent MW on urea-phosphate gels is also close to the predicted molecular weight.

Cellular localization

Mitochondrion membrane; Multi-pass membrane protein.

Alternative names

  • Mitochondrially encoded NADH dehydrogenase 6 antibody
  • MT ND6 antibody
  • mtND6 antibody
  • NADH dehydrogenase subunit 6 (complex I) antibody
  • NADH ubiquinone oxidoreductase chain 6 antibody
  • NADH Ubiquinone Oxidoreductase subunit ND6 antibody
  • NADH6 antibody
  • ND6 antibody
see all

Database links

Anti-NADH Dehydrogenase subunit 6 antibody images:

  Western blot - NADH Dehydrogenase subunit 6 antibody (ab81212)

Western blot - NADH Dehydrogenase subunit 6 antibody (ab81212)

Anti-NADH Dehydrogenase subunit 6 antibody (ab81212) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 19 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)


Gel concentration: 12%

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)

IHC image of ab81212 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81212, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)

This product has been referenced in:

  • Karamanlidis Get al. Defective DNA replication impairs mitochondrial biogenesis in human failing hearts. Circ Res 106:1541-8 (2010).Read more (PubMed: 20339121) »

See 1 publication for this product

Publishing research using ab81212? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"