Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)

Overview

• Product nameAnti-NADH Dehydrogenase subunit 6 antibody
• Description
  Rabbit polyclonal to NADH Dehydrogenase subunit 6
• Tested applicationsIHC-P, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Chimpanzee
• Immunogen

  Synthetic peptide corresponding to a region within internal sequence amino acids 110-159 (DGVVVVVNFN SVGSWMIYEG EGSGLIREDP IGAGALYDYG RWLVVVTGWT) of Human NADH Dehydrogenase subunit 6 (NP_536854).

  Run BLAST with Run BLAST with
• Positive controlHuman fetal lung lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex I

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Neuroscience
  • Sensory System
  • Visual system

Applications

Target

• RelevanceNADH Dehydrogenase subunit 6 (MTND6) is 1 of the 7 mitochondrial DNA (mtDNA) encoded subunits (MTND1, MTND2, MTND3, MTND4L, MTND4, MTND5, MTND6) included among the approximately 41 polypeptides of respiratory Complex I. Complex I accepts electrons from NADH, transfers them to ubiquinone (Coenzyme Q10), and uses the energy released to pump protons across the mitochondria inner membrane. MTND6 has been proposed to be a component of the iron-protein fragment. The predicted polypeptide has a molecular weight of 18.6 kD. Its apparent MW on SDS-polyacrylamide gels (PAGE) using Tris-glycine buffer is 16.7 kD, and the apparent MW on urea-phosphate gels is also close to the predicted molecular weight.
• Cellular localizationMitochondrion membrane; Multi-pass membrane protein.
• Database links
  • Entrez Gene: 4541 Human
  • Omim: 516006 Human
  • SwissProt: P03923 Human

• Alternative names
  Mitochondrially encoded NADH dehydrogenase 6 antibodyMT ND6 antibodymtND6 antibody

  NADH dehydrogenase subunit 6 (complex I) antibodyNADH ubiquinone oxidoreductase chain 6 antibodyNADH Ubiquinone Oxidoreductase subunit ND6 antibodyNADH6 antibodyND6 antibody

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Anti-NADH Dehydrogenase subunit 6 antibody images

• 

  Western blot - NADH Dehydrogenase subunit 6 antibody (ab81212)
  Anti-NADH Dehydrogenase subunit 6 antibody (ab81212) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 19 kDa
  Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADH Dehydrogenase subunit 6 antibody (ab81212)
  IHC image of ab81212 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81212, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.