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Western blot - NADH dehydrogenase subunit 4 antibody (ab26143)
(enlarge)
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Product Name
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NADH dehydrogenase subunit 4 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NADH dehydrogenase subunit 4
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human NADH dehydrogenase subunit 4.
(Peptide available as ab27767.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Glla
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.
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Cellular localization
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Mitochondrial
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Relevance
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NADH dehydrogenase subunit 4 is one of the seven mitochondrial DNA (mtDNA)-encoded subunits included among approximately 41 polypeptides of the respiratory complex I (NADH:ubiquinone oxidoreductase, EC 1.6.5.3), and is probably a component of the hydrophobic protein fragment. Complex I accepts electrons from NADH, transfers them to ubiquinone (coenzyme Q10) and uses the energy released to pump protons out across the mitochondrial inner membrane.
Defects in NADH dehydrogenase subunit 4 are a cause of Leber hereditary optic neuropathy, a maternally inherited disease resulting in acute bilateral blindness due to retinal degeneration and occuring predominantly in young men. Cardiac conduction defects and neurological defects have also been described, resulting in optic nerve degeneration and cardiac dysrhythmia. More recently NADH dehydrogenase subunit 4 has also been implicated in the progression of Parkinson's Disease.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.480 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NADH dehydrogenase subunit 4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NADH dehydrogenase subunit 4 antibody images, references, products related to ab26143 and other tools.
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NADH dehydrogenase subunit 4 antibody images:
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Search PubMed (MEDLINE) for references to NADH dehydrogenase subunit 4
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Western blot - NADH dehydrogenase subunit 4 antibody (ab26143)