NADP/NADPH Assay Kit (ab65349)
- Product nameNADP/NADPH Assay Kit
- Tests100 x 1 assay
- Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
- Assay typeQuantitative
- Assay time2h 00m
- Product overview
Abcam's NADP/NADPH Assay Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NAD+/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450nm.
Visit our FAQs page for tips and troubleshooting.
Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests NADP Cycling Buffer WM 1 x 15ml NADP Cycling Enzyme Mix Green 1 x 0.2ml NADP/NADPH Extraction Buffer NM 1 x 50ml NADPH Developer Purple 1 vial NADPH Standard (MW:833.36) Yellow 1 x 166.7µg Stop Solution Red 1 x 1.2ml
- Research Areas
Our Abpromise guarantee covers the use of ab65349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
NADP/NADPH Assay Kit images
NADPH Standard Curve. Assays were performed using various amounts of NADPH standard according to kit instructions. Solid square is NADPH. Diamond is NADH.
References for NADP/NADPH Assay Kit (ab65349)
This product has been referenced in:
- Gray JP et al. NAD kinase regulates the size of the NADPH pool and insulin secretion in pancreatic ß-cells. Am J Physiol Endocrinol Metab 303:E191-9 (2012). Functional Studies . Read more (PubMed: 22550069) »
- O'Neill JS & Reddy AB Circadian clocks in human red blood cells. Nature 469:498-503 (2011). Read more (PubMed: 21270888) »