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Anti-NAGA antibody
See all NAGA products (2) ...
Mouse polyclonal to NAGA
Reacts with
Human
Full length protein, corresponding to amino acids 1-411 of Human NAGA
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein A purified
Polyclonal
IgG
Western blot - NAGA antibody (ab67529)
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Our Abpromise guarantee covers the use of ab67529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
Detection Pair
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
NAGA encodes the lysosomal enzyme alpha N acetylgalactosaminidase, which cleaves alpha N acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease). Alpha galactosidase B was first found to be an isoenzyme of alpha galactosidases, but apparently it differs from alpha galactosidase A in substrate specificity and is alpha N acetylgalactosaminidase.
Lysosome
Western blot - NAGA antibody (ab67529)

All lanes : Anti-NAGA antibody (ab67529) at 1/500 dilution
Lane 1 : NAGA transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution
Predicted band size : 46 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
ab67529 has not yet been referenced specifically in any publications.
Publishing research using ab67529? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-NAGA antibody (ab67529) at 1/500 dilution
Lane 1 : NAGA transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution
Predicted band size : 46 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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