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Products:Neuroscience >> Sensory System >> Visual system
Overview
Product name
Anti-NAGLU antibody
Description
Rabbit polyclonal to NAGLU
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Cow, Chimpanzee, Rhesus monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAGLU.
(Peptide available as ab86400.)
Positive control
This antibody gave a positive signal in HepG2 Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> HSPGs
Neuroscience >> Sensory System >> Auditory system
Neuroscience >> Sensory System >> Visual system
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Target
Function
Involved in the degradation of heparan sulfate.
Tissue specificity
Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney.
Involvement in disease
Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
Cellular localization
Lysosome.
Target information above from: UniProt accessionP54802
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha N acetylglucosaminidase antibody
- alpha N acetylglucosaminidase, lysosomal antibody
- Alpha-N-acetylglucosaminidase 77 kDa form antibody
see all
Database links
- Entrez Gene: 4669 Human
- Entrez Gene: 27419 Mouse
- Omim: 609701 Human
- SwissProt: O54752 Human
- SwissProt: P54802 Human
- Unigene: 50727 Human
- Unigene: 95452 Mouse
Anti-NAGLU antibody images:
Western blot - NAGLU antibody (ab72178)
Anti-NAGLU antibody (ab72178) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 82 kDa
Immunocytochemistry/ Immunofluorescence - NAGLU antibody (ab72178)
ICC/IF image of ab72178 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72178, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 5ug/ml and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
References for Anti-NAGLU antibody (ab72178)
ab72178 has not yet been referenced specifically in any publications.
Publishing research using ab72178? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"