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Western blot - NAGLU antibody (ab72178)
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Immunocytochemistry/ Immunofluorescence - NAGLU antibody (ab72178)
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Product Name
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NAGLU antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NAGLU
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAGLU.
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Chmp, RMk
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in HepG2 Whole Cell Lysate
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Cellular localization
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Cytoplasmic (lysosomal)
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> HSPGs
Neuroscience >> Sensory System >> Auditory system
Neuroscience >> Sensory System >> Visual system
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Relevance
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The NAGLU gene product, alpha-N-acetylglucosaminidase, is a lysosomal protein that plays a role in the degradation of heparan sulphate. It hydrolyses terminal non-reducing N-acetyl-D-glucosamine moieties in N-acetyl-alpha-D-glucosaminides. Defects in the NAGLU gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B, which manifests as severe mental deterioration in childhood followed by death in the second decade. Symptoms include altered circadian rhythms and defects in motor function, vision, hearing and behaviour.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.600 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NAGLU antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NAGLU antibody images, references, products related to ab72178 and other tools.
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NAGLU antibody images:
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 Western blot - NAGLU antibody (ab72178)
NAGLU antibody (ab72178) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 82 kDa
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Immunocytochemistry/ Immunofluorescence - NAGLU antibody (ab72178)
ICC/IF image of ab72178 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72178, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 5ug/ml and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
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Search PubMed (MEDLINE) for references to NAGLU
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NAGLU antibody - more information
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Customer reviews (feedback) regarding NAGLU antibody |
Customer FAQs regarding NAGLU antibody |
Protocols for NAGLU antibody |
Price and availability of products related to NAGLU antibody |
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NAGLU antibody - related products:
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NAGLU antibody - other tools:
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