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Anti-NAGS antibody (ab65536)

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Overview

Product name

Anti-NAGS antibody

Description

Rabbit polyclonal to NAGS

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Dog, Pig

Immunogen

A region within synthetic peptide: PVLGGTPYLD KFVVSSSRQG QGSGQMLWEC LRRDLQTLFW RSRVTNPINP, corresponding to C terminal amino acids 434-483 of Human NAGS

PVLGGTPYLD KFVVSSSRQG QGSGQMLWEC LRRDLQTLFW RSRVTNPINP

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HepG2 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Amino Acids
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

  • Western blot - NAGS antibody (ab65536)Western blot - NAGS antibody (ab65536) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab65536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 58 kDa).

Target

Relevance

NAGS (N-acetylglutamate synthase) is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. It is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. Deficiencies in NAGS have been associated with hyperammonemia.

Cellular localization

Mitochondrion matrix.

Alternative names

  • AI415708 antibody
  • 1700120E20Rik antibody
  • AGAS antibody
  • Amino-acid acetyltransferase antibody
  • ARGA antibody
  • EC 2.3.1.1 antibody
  • MGC133025 antibody
  • N-acetylglutamate synthase antibody
  • N-acetylglutamate synthase, mitochondrial antibody
  • RP23-398F7.13 antibody
see all

Database links

Anti-NAGS antibody images:

  Western blot - NAGS antibody (ab65536)

Western blot - NAGS antibody (ab65536)

Anti-NAGS antibody (ab65536) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 58 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-NAGS antibody (ab65536)

ab65536 has not yet been referenced specifically in any publications.

Publishing research using ab65536? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"