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ab39795 |
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Read our guarantee »Publishing research using ab25968? Please let us know so that we can cite the reference in this datasheet
ab25968 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 160 kDa
Western blot analysis of NAIP in PC-3 cell lysate with anti-NAIP (CT) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Minor, lower molecular weight bands may represent alternately spliced forms.
ab25968 at 10µg/ml staining NAIP in A549 cells by ICC/IF
IHC image of ab25968 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-NAIP antibody (ab25968) at 1/1500 dilution + Mouse RAW 264.7 cell lysate at 20 µg
Secondary
HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 160 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa (possible non-specific binding).
Exposure time : 1 minute
This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappa
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