NAIP antibody (ab25968)

Product Name 

NAIP antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to NAIP

Immunogen 

Synthetic peptide, corresponding to 13 C terminal amino acids of Human NAIP (Genbank accession No. AAC52047).

Reacts with 
(species key)

Hu

Specificity 

ab25968 recognises Neuronal apoptosis inhibitor protein (NAIP).

Tested applications 
(see key)

ELISA, ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa). Can be blocked with NAIP peptide (ab39795).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

PC-3 cell lysate

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs

Relevance 

Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR); these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules. Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

See below for NAIP antibody images, references, products related to ab25968 and other tools.

NAIP antibody images:

References for NAIP antibody (ab25968)

  This product has been used in: (two most recent references)

Romanish MT et al. A novel protein isoform of the multicopy human NAIP gene derives from intragenic Alu SINE promoters. PLoS One 4:e5761 (2009). WB; Human. PubMed: 19488400

Hebb AL et al. Expression of the inhibitor of apoptosis protein family in multiple sclerosis reveals a potential immunomodulatory role during autoimmune mediated demyelination. Mult Scler 14:577-94 (2008). PubMed: 18566024
If you publish research using ab25968 please let us know so that we can cite the reference on this datasheet.

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