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Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-NAIP antibody
See all NAIP products (3) ...
Description
Rabbit polyclonal to NAIP
Specificity
ab25968 recognises Neuronal apoptosis inhibitor protein (NAIP).
Tested applications
WB, IHC-P, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to 13 C terminal amino acids of Human NAIP (Genbank accession No. AAC52047).
Positive control
PC-3 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).Can be blocked with NAIP peptide (ab39795).
IHC-P: Use at an assay dependent dilution. (PubMed: 20844241)
ELISA: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
Tissue specificity
Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
Sequence similarities
Contains 3 BIR repeats.
Contains 1 NACHT domain.
Target information above from: UniProt accessionQ13075
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Baculoviral IAP repeat containing 1 antibody
- Baculoviral IAP repeat-containing protein 1 antibody
- BIRC 1 antibody
see all
Database links
- Entrez Gene: 100510692 Human
- Entrez Gene: 4671 Human
- SwissProt: Q13075 Human
- Unigene: 654500 Human
- Unigene: 710305 Human
Anti-NAIP antibody images:
Western blot - NAIP antibody (ab25968)
Predicted band size : 160 kDa
Western blot analysis of NAIP in PC-3 cell lysate with anti-NAIP (CT) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Minor, lower molecular weight bands may represent alternately spliced forms.
Immunocytochemistry/ Immunofluorescence - NAIP antibody (ab25968)
ab25968 at 10µg/ml staining NAIP in A549 cells by ICC/IF
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NAIP antibody (ab25968)
IHC image of ab25968 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-NAIP antibody (ab25968)
This product has been referenced in:
- Petterson Tet al. Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors. J Leukoc Biol 89:177-87 (2011). IHC-P; Human.Read more (PubMed: 20844241) »
- Chiu HHet al. Induction of neuronal apoptosis inhibitory protein expression in response to androgen deprivation in prostate cancer. Cancer Lett 292:176-85 (2010). WB; Mouse.Read more (PubMed: 20044205) »
See all 4 publications for this product
Publishing research using ab25968? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"