![Western blot - NAK antibody [108A429] (ab12116) image](#NAK-Primary-antibodies-ab12116-1.jpg)
 image](#NAK-Primary-antibodies-ab12116-2.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-NAK antibody [108A429]
See all NAK products (10) ...
Description
Mouse monoclonal [108A429] to NAK
Specificity
ab12116 recognises human NAK.
Tested applications
Flow Cyt, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human).
Positive control
293 cell extracts transfected with human TBK1 cDNA.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Affinity purified.
Clonality
Monoclonal
Clone number
108A429
Isotype
IgG1
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 80 kDa (predicted molecular weight: 87.5 kDa).
IP: Use at an assay dependent dilution.
Target
Function
Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
Tissue specificity
Ubiquitous with higher expression in testis.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UHD2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 2.7.11.1 antibody
- FLJ11330 antibody
- NAK antibody
see all
Database links
- Entrez Gene: 29110 Human
- Omim: 604834 Human
- SwissProt: Q9UHD2 Human
- Unigene: 505874 Human
Anti-NAK antibody [108A429] images:
Western blot - NAK antibody [108A429] (ab12116)
![Western blot - NAK antibody [108A429] (ab12116)](/ps/datasheet/images/12/ab12116/NAK-Primary-antibodies-ab12116-1.jpg)
All lanes : Anti-NAK antibody [108A429] (ab12116) at 2 µg/ml
Lane 1 : 293 cells
Lane 2 : 293 cells transfected with Human NAK cDNA
Predicted band size : 87.5 kDa
Flow Cytometry-Anti-NAK antibody [108A429](ab12116)
](/ps/datasheet/images/12/ab12116/NAK-Primary-antibodies-ab12116-2.jpg)
Overlay histogram showing HeLa cells stained with ab12116 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12116, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-NAK antibody [108A429] (ab12116)
This product has been referenced in:
- Gao Let al. ABIN1 Protein Cooperates with TAX1BP1 and A20 Proteins to Inhibit Antiviral Signaling. J Biol Chem 286:36592-602 (2011). WB, IP.Read more (PubMed: 21885437) »
- Parvatiyar Ket al. TAX1BP1 and A20 inhibit antiviral signaling by targeting TBK1-IKKi kinases. J Biol Chem 285:14999-5009 (2010). WB, IP; Human.Read more (PubMed: 20304918) »
See all 2 publications for this product
Publishing research using ab12116? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"