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Immunocytochemistry/ Immunofluorescence - NAK antibody (ab8121)
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Product Name
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NAK antibody
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See all NAK antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NAK
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Immunogen
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Synthetic peptide (ERFGSLTMDGGLRNVD) corresponding to amino acids 712 to 727 of human NAK/TBK1, which are identical to those of mouse homologue.
BLAST 'ERFGSLTMDGGLRNVD' with  or 
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Reacts with
(species key)
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Hu, Ms
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Specificity
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No cross reactivity to IKKa,IKKb, IKKg, or IKKe.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 84 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MOLT-4 whole cell lysate
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Relevance
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NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor-associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family. Similar to IKK alpha and beta, NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumour promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536. NAK is also known to interact with TANK, TRAF-2 and TRIF.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NAK antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NAK antibody images, references, products related to ab8121 and other tools.
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NAK antibody images:
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 Immunocytochemistry/ Immunofluorescence - NAK antibody (ab8121)
ab8121 at 10µg/ml staining NAK in MOLT4 cells by ICC/IF
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References for NAK antibody (ab8121)
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This product has been used in:
McCoy CE et al. Glucocorticoids Inhibit IRF3 Phosphorylation in Response to Toll-like Receptor-3 and -4 by Targeting TBK1 Activation. J Biol Chem 283:14277-85 (2008). IP; Human. PubMed: 18356163
If you publish research using ab8121 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to NAK
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NAK antibody - more information
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Customer reviews (feedback) regarding NAK antibody |
Customer FAQs regarding NAK antibody |
Protocols for NAK antibody |
Price and availability of products related to NAK antibody |
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NAK antibody - related products:
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NAK antibody - other tools:
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Contact Abcam with a Technical Enquiry about NAK antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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