Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-NAK antibody
See all NAK products (10) ...
Description
Rabbit polyclonal to NAK
Specificity
No cross reactivity to IKKa,IKKb, IKKg, or IKKe.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide (ERFGSLTMDGGLRNVD) corresponding to amino acids 712 to 727 of human NAK/TBK1, which are identical to those of mouse homologue.
ERFGSLTMDG GLRNVD
Positive control
MOLT-4 whole cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
-
Immunocytochemistry/ Immunofluorescence - NAK antibody (ab8121)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 84 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
Tissue specificity
Ubiquitous with higher expression in testis.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UHD2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 2.7.11.1 antibody
- FLJ11330 antibody
- NAK antibody
see all
Database links
- Entrez Gene: 29110 Human
- Entrez Gene: 56480 Mouse
- Omim: 604834 Human
- SwissProt: Q9UHD2 Human
- SwissProt: Q9WUN2 Mouse
- Unigene: 505874 Human
- Unigene: 34580 Mouse
Anti-NAK antibody images:
Immunocytochemistry/ Immunofluorescence - NAK antibody (ab8121)
ab8121 at 10µg/ml staining NAK in MOLT4 cells by ICC/IF
References for Anti-NAK antibody (ab8121)
This product has been referenced in:
- McCoy CEet al. Glucocorticoids Inhibit IRF3 Phosphorylation in Response to Toll-like Receptor-3 and -4 by Targeting TBK1 Activation. J Biol Chem 283:14277-85 (2008). IP; Human.Read more (PubMed: 18356163) »
See 1 publication for this product
Publishing research using ab8121? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"