Recombinant Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free
See all NAK/TBK1 primary antibodies -
Description
Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172) - BSA and Azide free -
Host species
Rabbit -
Specificity
Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
-
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab206124) -
General notes
ab186469 is the carrier-free version of ab109272.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2867(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.Can be blocked with NAK/TBK1 (phospho S172) peptide (ab206124).
|
Notes |
---|
Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.Can be blocked with NAK/TBK1 (phospho S172) peptide (ab206124). |
Target
-
Function
Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively. -
Tissue specificity
Ubiquitous with higher expression in testis. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 29110 Human
- Omim: 604834 Human
- SwissProt: Q9UHD2 Human
- Unigene: 505874 Human
-
Alternative names
- EC 2.7.11.1 antibody
- FLJ11330 antibody
- FTDALS4 antibody
see all
Images
-
Dot blot analysis of NAK/TBK1 (phospho S172) phospho peptide (Lane 1) and NAK/TBK1 non-phospho peptide (Lane 2) labelling NAK/TBK1 (phospho S172) phospho peptide with ab109272 at a dilution of 1:1000 dilution (0.627μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.
Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% NFDM /TBST .This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109272).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (1)
ab186469 has been referenced in 1 publication.
- Han X et al. Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway. Open Med (Wars) 16:581-590 (2021). PubMed: 33869780