Anti-NALP2 antibody (ab36850)
Key features and details
- Rabbit polyclonal to NALP2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NALP2 antibody
See all NALP2 primary antibodies -
Description
Rabbit polyclonal to NALP2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to 12 amino acids near the carboxy-terminus of human NALP2.
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Positive control
- PC-3 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 121 kDa).
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 121 kDa). |
Target
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Function
Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
Tissue specificity
Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain. -
Sequence similarities
Belongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 8 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Domain
The DAPIN domain is necessary and sufficient for suppression of NFKB1 activation induced by TNF and for inducing IL1B secretion in collaboration with caspase-1. It is involved in interaction with PYCARD.
When isolated, the NACHT domain is involved in interaction with CARD8. This interaction is not detected for the full-length protein, maybe due to autoinhibition, this inhibition might by relieved by an inducible change in protein folding. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55655 Human
- Omim: 609364 Human
- SwissProt: Q9NX02 Human
- Unigene: 369279 Human
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Alternative names
- CLR19.9 antibody
- FLJ20510 antibody
- Leucine rich repeat and PYD containing protein 2 antibody
see all
Images
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Anti-NALP2 antibody (ab36850) at 1 µg/ml + PC-3 cell lysate
Predicted band size: 121 kDa
Observed band size: 96 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab36850 has been referenced in 2 publications.
- Brand FJ et al. Acidification changes affect the inflammasome in human nucleus pulposus cells. J Inflamm (Lond) 13:29 (2016). Human . PubMed: 27563282
- Ji S et al. Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. Infect Immun 77:1044-52 (2009). WB ; Human . PubMed: 19103770