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Product name
Anti-NALP2 antibody
See all NALP2 products (4) ...
Description
Rabbit polyclonal to NALP2
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to 12 amino acids near the carboxy-terminus of human NALP2.
Positive control
PC-3 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> CARD Family
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Western blot - NALP2 antibody (ab36850)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 121 kDa).
Target
Function
Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
Tissue specificity
Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.
Sequence similarities
Belongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 8 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
Domain
The DAPIN domain is necessary and sufficient for suppression of NFKB1 activation induced by TNF and for inducing IL1B secretion in collaboration with caspase-1. It is involved in interaction with PYCARD.
When isolated, the NACHT domain is involved in interaction with CARD8. This interaction is not detected for the full-length protein, maybe due to autoinhibition, this inhibition might by relieved by an inducible change in protein folding.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9NX02
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LRR and PYD domains-containing protein 2 antibody
- FLJ20510 antibody
- Leucine rich repeat and PYD containing protein 2 antibody
see all
Database links
- Entrez Gene: 55655 Human
- Entrez Gene: 232827 Mouse
- Omim: 609364 Human
- SwissProt: Q9NX02 Human
- Unigene: 369279 Human
- Unigene: 246598 Mouse
Anti-NALP2 antibody images:
Western blot - NALP2 antibody (ab36850)
Anti-NALP2 antibody (ab36850) at 1 µg/ml + PC-3 cell lysate
Predicted band size : 121 kDa
Observed band size : 96 kDa (why is the actual band size different from the predicted?)
References for Anti-NALP2 antibody (ab36850)
This product has been referenced in:
- Ji S et al. Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. Infect Immun 77:1044-52 (2009). WB; Human.Read more (PubMed: 19103770) »
See 1 publication for this product
Publishing research using ab36850? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"