AbcamBiochemicals

NAN-190 Fluorescent ligand (Red) (ab118177)

Overview

  • Product nameNAN-190 Fluorescent ligand (Red)
  • Description

    Fluorescent 5-HT1A antagonist

  • Biological descriptionFluorescent 5-HT1A antagonist (pKD values are 8.75 and 6.34 at 5-HT1A and 5-HT2A respectively). Wide range of applications which include localizing receptor distribution in tissues and cells, and live-cell imaging of receptor kinetics.

    Excitation wavelength: 633 nm

    Emission wavelength: 650 nm

  • Purity> 97%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab118177 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescent Cell Imaging Use at an assay dependent concentration.
    Small Molecule Fluorescent Optical Detection Use at an assay dependent concentration.
    Receptor Localization Use at an assay dependent concentration.

    NAN-190 Fluorescent ligand (Red) images

    • Left: ab118177 ligand (100 nM) binding to live CHO cells expressing serotonergic 5-HT1A receptors. Right: Binding blocked by the unlabelled competitor WAY-100635 (10 µM). Nuclei have been counter-stained with Hoechst.
    • To determine the antagonist activity of ab118177, serial dilutions of the agonist was added to wells with or without ab118177. The agonist Ca++ response in the absence or presence of ab118177 was determined by exciting at 340nm and 380 nm and ratioing the fluorescence intensity of Fura-2 signal collected at 320 nm. The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of ab118177, compared to the response curve for the agonist alone. KD values Antagonist –log; KD values 5-HT1A 8.75: 5-HT2A 6.34

    References for NAN-190 Fluorescent ligand (Red) (ab118177)

    ab118177 has not yet been referenced specifically in any publications.

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