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Western blot - NANOS2 antibody (ab70000)
(enlarge)
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Product Name
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NANOS2 antibody
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See all NANOS2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NANOS2
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Immunogen
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13 amino acid peptide near the carboxy terminus of the human NANOS2 (GenBank accession no. P60321).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab70000 does not cross-react with either Nanos1 or Nanos3.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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SK-N-SH cell lysate
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Research areas
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Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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Nanos is a zinc finger containing, RNA binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2 null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3 null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3 null background. Nanos3 expression can not rescue Nanos2-null defects.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NANOS2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NANOS2 antibody images, references, products related to ab70000 and other tools.
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NANOS2 antibody images:
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 Western blot - NANOS2 antibody (ab70000)
NANOS2 antibody (ab70000) at 2 µg/ml + SK-N-SH cell lysate at 15 µg
Predicted band size : 15 kDa
Observed band size : 15 kDa
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References for NANOS2 antibody (ab70000)
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This product has been used in:
Kusz KM et al. The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction. Mol Hum Reprod 15:165-71 (2009). WB, IHC-P; Human. PubMed: 19168545
If you publish research using ab70000 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to NANOS2
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NANOS2 antibody - more information
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Customer reviews (feedback) regarding NANOS2 antibody |
Customer FAQs regarding NANOS2 antibody |
Protocols for NANOS2 antibody |
Price and availability of products related to NANOS2 antibody |
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NANOS2 antibody - related products:
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NANOS2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NANOS2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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