Overview

  • Product name
  • Description
    Rabbit polyclonal to NANOS2
  • Specificity
    ab70000 does not cross-react with either Nanos1 or Nanos3.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IHC
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human NANOS2 (C terminal). 13 amino acid peptide near the carboxy terminus of the human NANOS2.

  • Positive control
    • SK-N-SH cell lysate, mouse brain, mouse spleen, A20, 3T3(NIH)
  • General notes

    Previous batches of ab70000 have been successfully used in IHC-P on testis tissue sections (please see Kusz KM et al., 2009). However, when tested on human brain tissue sections, ab70000 gave negative results in IHC-P (data not shown). We do not guarantee the current batch of ab70000 to work in IHC.

Properties

Applications

Our Abpromise guarantee covers the use of ab70000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
  • Application notes
    Is unsuitable for IHC.
  • Target

    • Function
      Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state.
    • Tissue specificity
      Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level).
    • Sequence similarities
      Belongs to the nanos family.
      Contains 1 nanos-type zinc finger.
    • Developmental stage
      Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level).
    • Domain
      The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
    • Cellular localization
      Cytoplasm. Cytoplasm > P-body. Cytoplasm > perinuclear region. Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis.
    • Information by UniProt
    • Database links
    • Alternative names
      • NANO2_HUMAN antibody
      • Nanos homolog 2 antibody
      • Nanos homolog 2 Drosophila antibody
      • NANOS2 antibody
      • NOS-2 antibody
      • NOS2 antibody
      see all

    Images

    • All lanes : Anti-NANOS2 antibody (ab70000)

      Lane 1 : Mouse Brain
      Lane 2 : Mouse Spleen
      Lane 3 : A20
      Lane 4 : 3T3(NIH)


      Predicted band size : 15 kDa
    • Anti-NANOS2 antibody (ab70000) at 2 µg/ml + SK-N-SH cell lysate at 15 µg

      Predicted band size : 15 kDa
      Observed band size : 15 kDa

    References

    This product has been referenced in:
    • Kong D  et al. PKA regulatory IIa subunit is essential for PGD2-mediated resolution of inflammation. J Exp Med 213:2209-26 (2016). Read more (PubMed: 27621415) »
    • Kusz KM  et al. The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction. Mol Hum Reprod 15:165-71 (2009). IHC-P, WB ; Human . Read more (PubMed: 19168545) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, embryonic day 15.5)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis, embryonic day 15.5
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Mr. Bryan Niedenberger

    Verified customer

    Submitted Apr 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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