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Anti-NANOS2 antibody (ab76568)

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Overview

Product name

Anti-NANOS2 antibody
See all NANOS2 products (2) ...

Description

Rabbit polyclonal to NANOS2

Tested applications

IHC-P, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

KLH conjugated synthetic peptide selected from the C terminal region of human NANOS2.

Positive control

Human testis tissue

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Ammonium Sulphate Precipitation

Purification notes

ab76568 is purified by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

Research areas

Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NANOS2 antibody - C-terminal (ab76568)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NANOS2 antibody - C-terminal (ab76568) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/10 - 1/50.

    IHC-P: 1/10 - 1/50.

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

Target

Function

Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state.

Tissue specificity

Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level).

Sequence similarities

Belongs to the nanos family.
Contains 1 nanos-type zinc finger.

Developmental stage

Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level).

Domain

The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.

Cellular localization

Cytoplasm. Cytoplasm > P-body. Cytoplasm > perinuclear region. Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis.

Target information above from: UniProt accessionP60321 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • NANO2_HUMAN antibody
  • Nanos homolog 2 antibody
  • Nanos homolog 2 Drosophila antibody
  • NANOS2 antibody
  • NOS-2 antibody
  • NOS2 antibody
see all

Database links

Anti-NANOS2 antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NANOS2 antibody - C-terminal (ab76568)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NANOS2 antibody - C-terminal (ab76568)

Formalin fixed and paraffin embedded human testis tissue stained with ab76568 at 1/10 dilution, followed by treatment with a peroxidase conjugated secondary antibody and DAB staining.

References for Anti-NANOS2 antibody (ab76568)

ab76568 has not yet been referenced specifically in any publications.

Publishing research using ab76568? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"