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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-NAP1L1 antibody
See all NAP1L1 products (4) ...
Description
Rabbit polyclonal to NAP1L1
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog, Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human NAP1L1.
(Peptide available as ab22417.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> INO80
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/250 - 1/1000.Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa).Can be blocked with NAP1L1 peptide (ab22417).
Target
Function
May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the nucleosome assembly protein (NAP) family.
Domain
The acidic domains are probably involved in the interaction with histones.
Post-translational
modifications
Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human.
Cellular localization
Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP55209
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NAP1L antibody
- FLJ16112 antibody
- hNRP antibody
see all
Database links
- Entrez Gene: 4673 Human
- Entrez Gene: 53605 Mouse
- Entrez Gene: 89825 Rat
- Entrez Gene: 380239 Xenopus laevis
- Omim: 164060 Human
- SwissProt: P55209 Human
- SwissProt: P28656 Mouse
- SwissProt: Q9Z2G8 Rat
see all
Anti-NAP1L1 antibody images:
Western blot - NAP1L1 antibody (ab21630)
Lanes 1 - 2 : Anti-NAP1L1 antibody (ab21630) at 1/1000 dilution
Lanes 3 - 4 : Anti-NAP1L1 antibody (ab21630) at 1/500 dilution
Lanes 5 - 6 : Anti-NAP1L1 antibody (ab21630) at 1/250 dilution
Lane 1 : U2OS cell lysate
Lane 2 : U2OS cell lysate treated with ionizing radiation
Lane 3 : U2OS cell lysate
Lane 4 : U2OS cell lysate treated with ionizing radiation
Lane 5 : U2OS cell lysate
Lane 6 : U2OS cell lysate treated with ionizing radiation
Performed under reducing conditions.
Predicted band size : 45 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 49 kDa (possible cleavage fragment,cross reactivity).
For each lane, U2OS cells, either treated or not treated with ionizing radiation, were scraped from a 60 mm dish and added to 75µl of 2X Laemmli buffer. 20µl of these samples were loaded into each lane. ab21630 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L1.
Western blot - NAP1L1 antibody (ab21630)
All lanes : Anti-NAP1L1 antibody (ab21630) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 20 µg
Lane 2 : A431 whole cell lysate at 20 µg
Lane 3 : HEK293 whole cell lysate at 20 µg
Lane 4 : HeLa whole cell lysate at 20 µg with NAP1L1 peptide (ab22417) at 1 µg/ml
Lane 5 : A431 whole cell lysate with NAP1L1 peptide (ab22417) at 1 µg/ml
Lane 6 : HEK293 whole cell lysate at 20 µg with NAP1L1 peptide (ab22417) at 1 µg/ml
Secondary
Alexa fluor goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size : 45 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa (possible cross reactivity, but this band is not blocked),49 kDa (possible cleavage fragment,cross reactivity).
ab21630 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L1. This band is competed away by the addition of the immunizing peptide, suggesting that this is a specific interaction.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody (ab21630)
IHC image of ab21630 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21630, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-NAP1L1 antibody (ab21630)
ab21630 has not yet been referenced specifically in any publications.
Publishing research using ab21630? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"