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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-NAP1L1 antibody
See all NAP1L1 products (4) ...
Description
Rabbit polyclonal to NAP1L1
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Dog, Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAP1L1.
(Peptide available as ab22418.)
Positive control
U2OS whole cell lysate HeLa whole cell lysate A431 whole cell lysate HEK293 whole cell lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> INO80
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/250 - 1/1000.Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa).Can be blocked with NAP1L1 peptide (ab22418).
Target
Function
May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the nucleosome assembly protein (NAP) family.
Domain
The acidic domains are probably involved in the interaction with histones.
Post-translational
modifications
Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human.
Cellular localization
Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP55209
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NAP1L antibody
- FLJ16112 antibody
- hNRP antibody
see all
Database links
- Entrez Gene: 417864 Chicken
- Entrez Gene: 4673 Human
- Entrez Gene: 53605 Mouse
- Entrez Gene: 89825 Rat
- Entrez Gene: 380239 Xenopus laevis
- Omim: 164060 Human
- SwissProt: P55209 Human
- SwissProt: P28656 Mouse
see all
Anti-NAP1L1 antibody images:
Western blot - NAP1L1 antibody (ab33076)
Lanes 1 - 2 : Anti-NAP1L1 antibody (ab33076) at 1/1000 dilution
Lanes 3 - 4 : Anti-NAP1L1 antibody (ab33076) at 1/500 dilution
Lanes 5 - 6 : Anti-NAP1L1 antibody (ab33076) at 1/250 dilution
Lane 1 : U2OS whole cell lysate
Lane 2 : U2OS whole cell lysate treated with ionizing radiation
Lane 3 : U2OS whole cell lysate
Lane 4 : U2OS whole cell lysate treated with ionizing radiation
Lane 5 : U2OS whole cell lysate
Lane 6 : U2OS whole cell lysate treated with ionizing radiation
Performed under reducing conditions.
Predicted band size : 45 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 49 kDa (possible cleavage fragment,cross reactivity),64 kDa (possible cross reactivity).
For each lane, U2OS cells, either treated or not treated with ionizing radiation, were scraped from a 60mm dish and added to 75µl of 2X Laemmli buffer. 20µl of these samples were loaded into each lane. ab33076 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L1.
Western blot - NAP1L1 antibody (ab33076)
All lanes : Anti-NAP1L1 antibody (ab33076) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HEK293 whole cell lysate
Lane 4 : HeLa whole cell lysate with NAP1L1 peptide (ab22418) at 1 µg/ml
Lane 5 : A431 whole cell lysate with NAP1L1 peptide (ab22418) at 1 µg/ml
Lane 6 : HEK293 whole cell lysate with NAP1L1 peptide (ab22418) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa fluor goat polyclonal to rabbit IgG (700) at 1/10000 dilution
developed using the ECL technique
Predicted band size : 45 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 49 kDa (possible cleavage fragment,cross reactivity),75 kDa (possible cross reactivity).
ab33076 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L1. This band is competed away by the addition of the immunizing peptide, suggesting that this is a specific interaction.
Western blot - NAP1L1 antibody (ab33076)
All lanes : Anti-NAP1L1 antibody (ab33076) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 45 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 74 kDa (possible cross reactivity).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody (ab33076)
IHC image of ab33076 staining in cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33076, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-NAP1L1 antibody (ab33076)
This product has been referenced in:
- Creyghton MPet al. H2AZ is enriched at polycomb complex target genes in ES cells and is necessary for lineage commitment. Cell 135:649-61 (2008). WB; Mouse.Read more (PubMed: 18992931) »
See 1 publication for this product
Publishing research using ab33076? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"