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Anti-NAP1L1 antibody (ab88580) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size : 45 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-NAP1L1 antibody (ab88580) at 1 µg/ml
Lane 1 : NAP1L1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 45 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
ab88580 at 10µg/ml staining NAP1L1 in HeLa cells by Immunofluorescence.
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