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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-NAP1L1 antibody
See all NAP1L1 products (4) ...
Description
Mouse polyclonal to NAP1L1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length Human NAP1L1 (NP_004528.1, 1-391aa).
Positive control
NAP1L1 transfected 293T cell lysate, HeLa cells and cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> INO80
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 45 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the nucleosome assembly protein (NAP) family.
Domain
The acidic domains are probably involved in the interaction with histones.
Post-translational
modifications
Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human.
Cellular localization
Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP55209
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NAP1L antibody
- FLJ16112 antibody
- hNRP antibody
see all
Database links
- Entrez Gene: 4673 Human
- Omim: 164060 Human
- SwissProt: P55209 Human
- Unigene: 524599 Human
- Unigene: 695185 Human
Anti-NAP1L1 antibody images:
Western blot - NAP1L1 antibody (ab88580)
Anti-NAP1L1 antibody (ab88580) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size : 45 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Western blot - NAP1L1 antibody (ab88580)
All lanes : Anti-NAP1L1 antibody (ab88580) at 1 µg/ml
Lane 1 : NAP1L1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 45 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - NAP1L1 antibody (ab88580)
ab88580 at 10µg/ml staining NAP1L1 in HeLa cells by Immunofluorescence.
References for Anti-NAP1L1 antibody (ab88580)
ab88580 has not yet been referenced specifically in any publications.
Publishing research using ab88580? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"