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All lanes : Anti-NAP1L3 antibody (ab70205) at 1/500 dilution
Lane 1 : NAP1L3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 58 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
ab70205 at 10µg/ml staining NAP1L3 in Hela cells by Immunocytochemistry/Immunofluorescence.
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