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Products:Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Other Nuclear Bodies
Overview
Product name
Anti-NAP1L3 antibody
Description
Mouse polyclonal to NAP1L3
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length human NAP1L3 protein (AAH34954.1)
Positive control
NAP1L3 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Chromatin assembly
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Other Nuclear Bodies
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
WB: 1/500 - 1/1000.Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).
Target
Relevance
Belongs to the nucleosome assembly protein (NAP) family.
Cellular localization
Nuclear
Alternative names
- BNAP antibody
- Nucleosome assembly protein 1 like 3 antibody
Database links
- Entrez Gene: 4675 Human
- Omim: 300117 Human
- SwissProt: Q99457 Human
Anti-NAP1L3 antibody images:
Western blot - NAP1L3 antibody (ab70205)
All lanes : Anti-NAP1L3 antibody (ab70205) at 1/500 dilution
Lane 1 : NAP1L3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 58 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - NAP1L3 antibody (ab70205)
ab70205 at 10µg/ml staining NAP1L3 in Hela cells by Immunocytochemistry/Immunofluorescence.
References for Anti-NAP1L3 antibody (ab70205)
ab70205 has not yet been referenced specifically in any publications.
Publishing research using ab70205? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"