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Anti-NAPE-PLD antibody (ab77474)

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Overview

Product name

Anti-NAPE-PLD antibody
See all NAPE-PLD products (5) ...

Description

Rabbit polyclonal to NAPE-PLD

Tested applications

WB, ICC, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide derived from within amino acids 100-200 of Mouse NAPE-PLD (SwissProt Q8BH82)

Positive control

DU145 cell extracts; Mouse brain tissue lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other

  • Western blot - NAPE-PLD antibody (ab77474)Western blot - NAPE-PLD antibody (ab77474) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab77474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/2500 - 1/5000.Pred...Read more →

    WB: 1/2500 - 1/5000.Predicted molecular weight: 46 kDa.

  • ICC

     ICC: 1/1000 - 1/5000.

    ICC: 1/1000 - 1/5000.

  • ICC/IF

     ICC/IF: 1/1000 - 1/5000.

    ICC/IF: 1/1000 - 1/5000.

Target

Function

Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors.

Sequence similarities

Belongs to the NAPE-PLD family.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ6IQ20 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • C7orf18 antibody
  • FMP30 antibody
  • Mbldc1 antibody
  • N acyl phosphatidylethanolamine hydrolyzing phospholipase D antibody
  • N acyl phosphatidylethanolamine phospholipase D antibody
  • N-acyl phosphatidylethanolamine phospholipase D antibody
  • N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D antibody
  • NAPE hydrolyzing phospholipase D antibody
  • NAPE-hydrolyzing phospholipase D antibody
  • NAPE-PLD antibody
  • NAPEP_HUMAN antibody
  • NAPEPLD antibody
see all

Database links

see all

Anti-NAPE-PLD antibody images:

  Western blot - NAPE-PLD antibody (ab77474)

Western blot - NAPE-PLD antibody (ab77474)

Anti-NAPE-PLD antibody (ab77474) at 1/5000 dilution + DU145 cell extracts

Predicted band size : 46 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-NAPE-PLD antibody (ab77474)

This product has been referenced in:

  • Srisai Det al. Characterization of the Hyperphagic Response to Dietary Fat in the MC4R Knockout Mouse. Endocrinology 152:890-902 (2011). WB; Mouse.Read more (PubMed: 21239438) »

See 1 publication for this product

Publishing research using ab77474? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"