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Western blot - NAPE-PLD antibody (ab77474)
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Product Name
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NAPE-PLD antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NAPE-PLD
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Immunogen
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Synthetic peptide derived from within amino acids 100-200 of Mouse NAPE-PLD (SwissProt Q8BH82)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/1000 - 1/5000.
ICC/IF: 1/1000 - 1/5000.
WB: 1/2500 - 1/5000. Predicted molecular weight: 46 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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DU145 cell extracts; Mouse brain tissue lysate.
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Cellular localization
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Membrane
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Research areas
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Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
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Relevance
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N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme which releases the endocannabinoid anandamide and other bioactive N-acylethanolamines from their corresponding NAPEs in animal tissues. NAPE-PLD has been shown to be expressed by specific populations of neurons in the brain and targeted to axonal processes. Recent studies have suggested that NAEs generated by NAPE-PLD in axons may act as anterograde synaptic signaling molecules that regulate the activity of postsynaptic neurons. NAPE-PLD has also been shown to catalyze the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a natural lipid amide that inhibits food intake in free-feeding rodents by prolonging latency to feed and postmeal interval.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, Whole serum Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NAPE-PLD antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NAPE-PLD antibody images, references, products related to ab77474 and other tools.
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NAPE-PLD antibody images:
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Search PubMed (MEDLINE) for references to NAPE-PLD
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NAPE-PLD antibody - more information
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Customer reviews (feedback) regarding NAPE-PLD antibody |
Customer FAQs regarding NAPE-PLD antibody |
Protocols for NAPE-PLD antibody |
Price and availability of products related to NAPE-PLD antibody |
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NAPE-PLD antibody - related products:
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NAPE-PLD antibody - other tools:
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Contact Abcam with a Technical Enquiry about NAPE-PLD antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - NAPE-PLD antibody (ab77474)