Anti-NAPRT1 antibody (ab127699)
Key features and details
- Rabbit polyclonal to NAPRT1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NAPRT1 antibody
See all NAPRT1 primary antibodies -
Description
Rabbit polyclonal to NAPRT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 107-408 of Human NAPRT1.
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Positive control
- A549, HeLa, HepG2 and HCT116 whole cell lysates; Human lung carcinoma tissue, HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 58 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. -
Sequence similarities
Belongs to the NAPRTase family. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 93100 Human
- Omim: 611552 Human
- SwissProt: Q6XQN6 Human
- Unigene: 493164 Human
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Alternative names
- FHA HIT interacting protein antibody
- FHA-HIT-interacting protein antibody
- FHIP antibody
see all
Images
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Anti-NAPRT1 antibody (ab127699) at 1/10000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS PAGE -
ab127699, at a dilution of 1/500, staining NAPRT1 in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.
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ab127699, at a dilution of 1/500, staining NAPRT1 in methanol-fixed HeLa cells by Immunofluorescence (Green). Alpha-tubulin filaments were stained Red.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab127699 has been referenced in 1 publication.
- James EL et al. Replicatively senescent human fibroblasts reveal a distinct intracellular metabolic profile with alterations in NAD+ and nicotinamide metabolism. Sci Rep 6:38489 (2016). PubMed: 27924925