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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Overview
Product name
Anti-NARS antibody - C-terminal
See all NARS products (5) ...
Description
Rabbit polyclonal to NARS - C-terminal
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C terminal region of human NARS conjugated to KLH
Positive control
Jurkat cell line lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
ab71478 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10 - 1/50.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/50 - 1/100.Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Target
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO43776
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytoplasmic antibody
- 3010001M15Rik antibody
- AA960128 antibody
see all
Database links
- Entrez Gene: 4677 Human
- Omim: 108410 Human
- SwissProt: O43776 Human
- Unigene: 465224 Human
Anti-NARS antibody - C-terminal images:
Western blot - NARS antibody - C-terminal (ab71478)
Anti-NARS antibody - C-terminal (ab71478) at 1/60 dilution + Jurkat cell line lysate at 35 µg
Predicted band size : 63 kDa
Observed band size : 63 kDa
Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NARS antibody - C-terminal(ab71478)
ab71478 at 1/50 dilution staining NARS - C - terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-NARS antibody - C-terminal (ab71478)
ab71478 has not yet been referenced specifically in any publications.
Publishing research using ab71478? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"