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Anti-NARS antibody [NARS2G5] (ab53473) at 1/100 dilution + NIH 3T3 whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
developed using the ECL technique
Predicted band size : 63 kDa
Observed band size : 63 kDa
ab53473 at 5ug used to immunoprecipitate RIPA lysate of HT-1080 cells (500µg). Western blot used ab53473 at 1/50 dilution with anti-mouse IgG at 1/2500 as secondary.
ab53473 at 1/10 dilution staining NARS in HT-1080 cells, by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 used as secondary.
Anti-NARS antibody [NARS2G5] (ab53473) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg
Secondary
anti-mouse IgG at 1/2500 dilution
developed using the ECL technique
Predicted band size : 63 kDa
Observed band size : 63 kDa
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