![Western blot - NARS antibody [NARS2G5] (ab53473) image](#ab53473wbnih.gif)
![Immunoprecipitation - NARS antibody [NARS2G5] (ab53473) image](#ab53473ip.gif)
![Immunocytochemistry - NARS antibody [NARS2G5] (ab53473) image](#ab53473icc.gif)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Overview
Product name
Anti-NARS antibody [NARS2G5]
See all NARS products (5) ...
Description
Mouse monoclonal [NARS2G5] to NARS
Tested applications
WB, IP, ICC, Dot Blotmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant fragment from Human NARS.
Positive control
HT-1080 cells and whole cell lysate, NIH 3T3 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab53473 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Clonality
Monoclonal
Clone number
NARS2G5
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP: Use at 10 µg/mg of lysate.
ICC: 1/10
Dot: Use at an assay dependent dilution.
Target
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO43776
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytoplasmic antibody
- 3010001M15Rik antibody
- AA960128 antibody
see all
Database links
- Entrez Gene: 4677 Human
- Entrez Gene: 70223 Mouse
- Omim: 108410 Human
- SwissProt: O43776 Human
- SwissProt: Q3T9A7 Mouse
- SwissProt: Q8BP47 Mouse
- Unigene: 465224 Human
- Unigene: 482137 Mouse
Anti-NARS antibody [NARS2G5] images:
Western blot - NARS antibody [NARS2G5] (ab53473)
![Western blot - NARS antibody [NARS2G5] (ab53473)](/ps/datasheet/Images/53/ab53473/ab53473wbnih.gif)
Anti-NARS antibody [NARS2G5] (ab53473) at 1/100 dilution + NIH 3T3 whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
developed using the ECL technique
Predicted band size : 63 kDa
Observed band size : 63 kDa
Immunoprecipitation - NARS antibody [NARS2G5] (ab53473)
![Immunoprecipitation - NARS antibody [NARS2G5] (ab53473)](/ps/datasheet/Images/53/ab53473/ab53473ip.gif)
ab53473 at 5ug used to immunoprecipitate RIPA lysate of HT-1080 cells (500µg). Western blot used ab53473 at 1/50 dilution with anti-mouse IgG at 1/2500 as secondary.
Immunocytochemistry - NARS antibody [NARS2G5] (ab53473)
![Immunocytochemistry - NARS antibody [NARS2G5] (ab53473)](/ps/datasheet/Images/53/ab53473/ab53473icc.gif)
ab53473 at 1/10 dilution staining NARS in HT-1080 cells, by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 used as secondary.
Western blot - NARS antibody [NARS2G5] (ab53473)
![Western blot - NARS antibody [NARS2G5] (ab53473)](/ps/datasheet/Images/53/ab53473/ab53473wbht.gif)
Anti-NARS antibody [NARS2G5] (ab53473) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg
Secondary
anti-mouse IgG at 1/2500 dilution
developed using the ECL technique
Predicted band size : 63 kDa
Observed band size : 63 kDa
References for Anti-NARS antibody [NARS2G5] (ab53473)
ab53473 has not yet been referenced specifically in any publications.
Publishing research using ab53473? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"