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Anti-NARS antibody (ab50144) at 1/500 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml
Secondary
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 63 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
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