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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Overview
Product name
Anti-NARS antibody
See all NARS products (5) ...
Description
Rabbit polyclonal to NARS
Specificity
This antibody reacts specifically with 63kDa NARS protein.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein (Human)
Positive control
HEK293 cells lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
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Western blot - NARS antibody (ab50144)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP: Use at an assay dependent dilution.
Target
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO43776
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytoplasmic antibody
- 3010001M15Rik antibody
- AA960128 antibody
see all
Database links
- Entrez Gene: 4677 Human
- Entrez Gene: 70223 Mouse
- Omim: 108410 Human
- SwissProt: O43776 Human
- SwissProt: Q3T9A7 Mouse
- SwissProt: Q8BP47 Mouse
- Unigene: 465224 Human
- Unigene: 482137 Mouse
Anti-NARS antibody images:
Western blot - NARS antibody (ab50144)
Anti-NARS antibody (ab50144) at 1/500 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml
Secondary
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 63 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
References for Anti-NARS antibody (ab50144)
ab50144 has not yet been referenced specifically in any publications.
Publishing research using ab50144? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"