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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-NARS2 antibody - C-terminal
See all NARS2 products (2) ...
Description
Rabbit polyclonal to NARS2 - C-terminal
Tested applications
IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C-terminal region of human NARS2 conjugated to KLH
Positive control
HeLa cell line lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
ab72327 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Mito. Translation
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100.Detects a band of approximately 47 kDa (predicted molecular weight: 54 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Relevance
NARS2 (Asparaginyl tRNA synthetase 2) belongs to the class-II aminoacyl-tRNA synthetase family.
Cellular localization
Mitochondrion matrix.
Alternative names
- MGC125113 antibody
- RGD1305145 antibody
- AI875199 antibody
see all
Database links
- Entrez Gene: 79731 Human
- SwissProt: Q96I59 Human
Anti-NARS2 antibody - C-terminal images:
Western blot - NARS2 antibody - C-terminal (ab72327)
Anti-NARS2 antibody - C-terminal (ab72327) at 1/60 dilution + HeLa cell line lysate at 35 µg
Predicted band size : 54 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NARS2 antibody - C-terminal(ab72327)
ab72327 at 1/50 dilution staining NARS2 - C - terminal in human prostate carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-NARS2 antibody - C-terminal (ab72327)
ab72327 has not yet been referenced specifically in any publications.
Publishing research using ab72327? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"