|
|
|
|
Western blot - NASP antibody (ab72494)
(enlarge)
|
|
|
|
|
Product Name
|
NASP antibody
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to NASP
|
|
Immunogen
|
Synthetic peptide corresponding to a region between residues 150 and 200 of human NASP (BAD96536.1)
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: 1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 86 kDa).
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa and 293T whole cell lysates.
|
|
Cellular localization
|
Cytoplasmic and Nuclear
|
|
Research areas
|
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
|
|
Relevance
|
NASP, Nuclear autoantigenic sperm protein, is a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
ab72494 was affinity purified using an epitope specific to NASP immobilized on solid support.
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NASP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for NASP antibody images, references, products related to ab72494 and other tools.
|
| |
NASP antibody images:
|
 Western blot - NASP antibody (ab72494)
All lanes : NASP antibody (ab72494) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 86 kDa
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
|
| |
| |
|
Search PubMed (MEDLINE) for references to NASP
|
| |
NASP antibody - more information
|
Customer reviews (feedback) regarding NASP antibody |
Customer FAQs regarding NASP antibody |
Protocols for NASP antibody |
Price and availability of products related to NASP antibody |
| |
NASP antibody - related products:
|
|
|
| |
NASP antibody - other tools:
|
Contact Abcam with a Technical Enquiry about NASP antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
