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Overview
Product name
Anti-NASP antibody
See all NASP products (3) ...
Description
Rabbit polyclonal to NASP
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 150 and 200 of human NASP (BAD96536.1)
Positive control
HeLa and 293T whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab72494 was affinity purified using an epitope specific to NASP immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
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Western blot - NASP antibody (ab72494)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 160 kDa (predicted molecular weight: 86 kDa).
Application notes
Is unsuitable for or IP.
Target
Function
Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
Tissue specificity
Isoform 1 is testis- and sperm-specific.
Sequence similarities
Belongs to the NASP family.
Contains 3 TPR repeats.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP49321
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5033430J04Rik antibody
- AI131596 antibody
- AI317140 antibody
see all
Database links
- Entrez Gene: 4678 Human
- Omim: 603185 Human
- SwissProt: P49321 Human
- Unigene: 319334 Human
Anti-NASP antibody images:
Western blot - NASP antibody (ab72494)
All lanes : Anti-NASP antibody (ab72494) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 86 kDa
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
References for Anti-NASP antibody (ab72494)
This product has been referenced in:
- Li Fet al. Identification and analysis of signaling networks potentially involved in breast carcinoma metastasis to the brain. PLoS One 6:e21977 (2011). WB; Human.Read more (PubMed: 21779361) »
- Grad Iet al. The Molecular Chaperone Hsp90a Is Required for Meiotic Progression of Spermatocytes beyond Pachytene in the Mouse. PLoS One 5:e15770 (2010). WB; Mouse.Read more (PubMed: 21209834) »
See all 2 publications for this product
Publishing research using ab72494? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"