NAT2 protein (Active) (ab54272)

Product Name 

NAT2 protein (Active)

Product type 

Proteins and Peptides

Peptide 

Recombinant full length Human NAT2 (derived from the wild type NAT2*4 allele) and expressed in baculovirus infected BTI insect cells.

Specificity 

Unit Definition: One unit will acetylate one pmole of sulfamethazine per minute at pH 7.5 at 37 °C.

Tested applications 
(see key)

FuncS

Application notes 
(see key)

Recommended dilutions
N-acetyl transferase 2 Activity:
Incubations were conducted at 0.5 µg/ml protein in 50 mM triethanolamine (pH 7.5), 1 mM EDTA, 1 mM DTT, 4.6 mM acetyl-d,l-carnitine, 0.06 U/ml carnitine acetyltransferase, 0.1 mM acetyl coenzyme A, and 40 µM sulfamethazine as substrate for 15 minutes at 37 °C. The reaction is stopped by the addition of 0.025 ml of 6 % acetic acid/94% acetonitrile.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Signal Transduction >> Metabolism >> Drug metabolism

Relevance 

NAT2 participates in the detoxification of a plethora of hydrazine and arylamine drugs. It catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens.


NAT2 is derived from a soluble cell fraction from insect cells (Trichopulsi ni, BTI-TN-5B1-4) expressing human NAT2 wild-type cDNA using a baculovirus expression system.

Database links 

Storage buffer 

Preservative: None
Constituents: 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 7.5

Purification notes 

Purified by affinity purification.

Form 

Liquid

Concentration 

2.500 mg/ml

Storage instructions 

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

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