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Anti-NAT9 antibody (ab68964) at 1/500 dilution + human liver lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 23 kDa
Observed band size : 23 kDa
All lanes : Anti-NAT9 antibody (ab68964) at 1/500 dilution
Lane 1 : transfected 293T cell lysate
Lane 2 : untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 23 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
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