Recombinant Anti-NBL1 antibody [EPR12397] (ab174843)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12397] to NBL1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NBL1 antibody [EPR12397]
See all NBL1 primary antibodies -
Description
Rabbit monoclonal [EPR12397] to NBL1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
Unsuitable for: IHC or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A431 and Human prostate lysates; A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12397 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 19 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 19 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 100532736 Human
- Entrez Gene: 4681 Human
- Omim: 600613 Human
- SwissProt: P41271 Human
- Unigene: 466662 Human
- Unigene: 654502 Human
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Alternative names
- D1S1733E antibody
- DAN antibody
- DAN domain family member 1 antibody
see all
Images
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All lanes : Anti-NBL1 antibody [EPR12397] (ab174843) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Human prostate lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa -
Immunofluorescent analysis of A431 cells labeling NBL1 with ab174843 at 1/100 dilution (red). DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized A431 cells labeling NBL1 with ab174843 at 1/10 dilution (red), or negative control rabbit IgG (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab174843 has not yet been referenced specifically in any publications.