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Overview

  • Product nameNBL1 Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 35 pg/ml
  • Range
    27.43 pg/ml - 20000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 111.3 107% - 122%
    Serum 100.1 91% - 109%
    Plasma 81.82 72% - 90%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's Human NBL1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NBL1 in serum, plasma and cell culture supernatants.

     

    This assay employs an antibody specific for Human NBL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NBL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NBL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionPossible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
  • Tissue specificityMost abundant in normal lung and meningioma.
  • Sequence similaritiesBelongs to the DAN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P41271 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        DANDAN domain family member 1DAND 1
        DAND1Differential screening selected gene aberrant in neuroblastomaMGC8972NB NBL 1NBL1NBL1_HUMANNeuroblastoma candidate region suppression of tumorigenicity 1Neuroblastoma suppression of tumorigenicity 1Neuroblastoma suppressor of tumorigenicity 1NO 3NO3Protein N03Zinc finger protein DAN
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99997 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      NBL1 Human ELISA Kit images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99997

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99997

      Protocols

      References for NBL1 Human ELISA Kit (ab99997)

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