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Product name
Anti-NBR1 antibody
See all NBR1 products (2) ...
Description
Mouse monoclonal to NBR1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant fragment: EPQVTLNVTF KNEIQSFLVS DPENTTWADI EAMVKVSFDL NTIQIKYLDE ENEEVSINSQ GEYEEALKMA VKQGNQLQMQ VHEGHHVVDE APPPV, corresponding to amino acids 2-97 of Human NBR1
EPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDL NTIQIKYLDEENEEVSINSQGEYEEALKMAVKQGNQLQMQ VHEGHHVVDEAPPPV
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.
IHC-P: Use a concentration of 4 µg/ml.
Target
Function
Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.
Sequence similarities
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger.
Domain
The OPR domain mediates interaction with SQSTM1.
Cellular localization
Cytoplasm. Cytoplasmic vesicle > autophagosome. Lysosome. Cytoplasm > myofibril > sarcomere > M line. In cardiac muscles localizes to the sarcomeric M line (By similarity). Is targeted to lysosomes for degradation.
Target information above from: UniProt accessionQ14596
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1A1 3B antibody
- 1A13B antibody
- Cell migration-inducing gene 19 protein antibody
see all
Database links
- Entrez Gene: 4077 Human
- Entrez Gene: 17966 Mouse
- Omim: 166945 Human
- SwissProt: Q14596 Human
- SwissProt: P97432 Mouse
- Unigene: 277721 Human
- Unigene: 708158 Human
- Unigene: 784 Mouse
Anti-NBR1 antibody images:
Western blot - NBR1 antibody (ab55474)
Western blot against tagged recombinant protein immunogen using ab55474 NBR1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NBR1 antibody (ab55474)
ab55474 (4µg/ml) staining NBR1 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is moderate cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-NBR1 antibody (ab55474)
This product has been referenced in:
- Perera S et al. Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover. Dev Biol 351:46-61 (2011). WB; Mouse.Read more (PubMed: 21185285) »
- Whitehouse CA et al. Neighbor of Brca1 gene (Nbr1) functions as a negative regulator of postnatal osteoblastic bone formation and p38 MAPK activity. Proc Natl Acad Sci U S A 107:12913-8 (2010).Read more (PubMed: 20616007) »
See all 2 publications for this product
Publishing research using ab55474? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"